Fix 1: Troubleshoot the hardware issue
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The middle mouse button not working issue might be due to the hardware problem so you can check the mouse and see if the problem fixes or not.
For specifically locating the issue, you can unplug the mouse from the current computer and plug it to another computer and check if the middle button works or not.
If the mouse works on another computer then it might be the connection issue of your computer and your mouse or it might be the software issue that has caused the mouse to stop working.
If the middle mouse button is still not working on another computer as well then it can be the hardware problem with the mouse. You can open the mouse and check the mouse components if you have knowledge about it.
Fix 2: Run the Hardware troubleshooter
Source :- lynda.com
The built-in hardware and device troubleshooter can help you troubleshoot the device hardware issue.
The first step for fixing the problem with this solution is to open your control panel and click Troubleshooting.
Now, click hardware and sound.
Now, click hardware and devices.
Now, click next for starting troubleshooting. Follow the given instructions to finish.
Now, restart your computer and see if the middle mouse button is working or not.
If this solution is also not working, then you can try out the next solution.
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Fix 3: Update the mouse driver
Source :- lynda.com
The outdated or missing mouse driver can lead to the middle mouse button not working so you should update the mouse driver to the latest version for solving the problem.
You can download the latest version of the mouse driver file from the manufacturer and install on your pc. There are some tools available for automatically updating the required mouse drivers.
Try out all the solutions given above and see if your mouse middle button works or not. We ensure your problem will get solved very soon.